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HTLV-induced cell fusion in the presence of glut-1. 293T Ω12 cells (stably express LacZω protein) were transfected with the indicated glycoproteins and then cocultured with the indicated cells stably expressing LacZα peptide. After 48 h, cells were lysed and soluble β-galactosidase activity was measured. Note the increase in the activity of HTLV-1 env in the presence of glut-1; no increase was observed when 293T cells were transfected or when LacZα peptide was absent.
