FIG. 5.

(A) Disassembly of actin stress fibers in CEC infected with BAC20 at 48 h p.i. Cells were fixed with 90% acetone at 48 h after infection. MDV proteins were detected with polyclonal convalescent-phase serum that was visualized using an Alexa 568-conjugated anti-chicken IgG as the secondary antibody (red). Actin was visualized with phalloidin-Alexa 488 (green). Cells were finally inspected using a Zeiss Axiovert fluorescence microscope, and pictures were taken with a 40× objective before processing with Adobe Photoshop. (B) Percentage of infected cells with intact and depolymerized actin stress fibers. A total of 300 infected cells in three independent experiments were inspected.