FIG. 6.
(A) Subcellular localization of pUS3* in CEC transfected with expression plasmid pcUS3*. Cells were fixed at the given time points with 90% acetone, and FLAG-tagged US3 (red) was detected using the anti-FLAG M2 antibody and Alexa 568-conjugated anti-mouse IgG as the secondary antibody. Actin (green) was stained with phalloidin-Alexa 488. Red and green fluorescence signals were recorded separately by using appropriate filters with a confocal microscope (Olympus). Overlay of the pUS3 and actin fluorescent signals is shown in the merge. (B) Percentages of pUS3*- and pcMgB-transfected cells with intact actin stress fibers (100 cells were scored) at 24 and 48 h after transfection. Percentages represent means and standard deviations of three independent experiments.