FIG. 2.

Recognition of mutant ΔgB₆₅₁ by AD-1-specific MAb 7-17. Wild-type (wt) gB and mutant ΔgB651 were expressed in monkey kidney cells infected with recombinant vaccinia viruses as described in Materials and Methods. (A) Following labeling with [³⁵S]Met/Cys, infected cell proteins were immune precipitated with the AD-1-specific MAb 7-17, the gB oligomer-specific antibody 27-39, or antibody 58-15, which is directed at an epitope located at the extreme carboxyl terminus of gB, and the precipitated proteins were analyzed by SDS-PAGE under reducing conditions. The migration of the 150-kDa gB precursor protein of gB and the 120-kDa precursor protein of ΔgB₆₅₁ is indicated in the margin. The migration of the 55-kDa TM cleavage product of gB and the 30-kDa cleavage product of the ΔgB₆₅₁ mutant are also indicated. Note that ΔgB₆₅₁ was not precipitated by MAb 58-15. (B) Supernatant from the infected cells was centrifuged at 13,000 × g for 20 min and then precipitated with MAb 7-17. Precipitated proteins were analyzed by SDS-PAGE as described above.