FIG. 3.

Oligomerization of ΔgB₆₅₁. Monkey kidney cells were infected with a recombinant vaccinia virus expressing ΔgB₆₅₁ and pulse-labeled with [³⁵S]Met/Cys for 10 min. The cultures were then washed extensively and either harvested immediately (time [T] = 0 min) or incubated in media for 120 min. Infected cell proteins from both cultures were centrifuged through a 5 to 30% sucrose gradient for 16 h as described in Materials and Methods. One-milliliter fractions were collected from the bottom of the gradient and precipitated with MAb 7-17. Precipitated proteins were analyzed by SDS-PAGE. Note the increased concentration of precipitated ΔgB₆₅₁ in fraction 3 in the later chase interval, indicating multimerization. The top of the gradient is indicated.