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. Author manuscript; available in PMC: 2024 Jun 15.
Published in final edited form as: J Immunol. 2023 Jun 15;210(12):2016–2028. doi: 10.4049/jimmunol.2200822

Figure 6. BALF protein levels of proinflammatory cytokines associated with dysregulated chemokines increased following LPS-induced ALI.

Figure 6.

BALF was taken from the lungs of mice to perform ELISAs to screen for proinflammatory cytokines associated with immune cell trafficking. Each group had 8 mice (n=8), and the experiment was carried out once. A. BALF protein levels of IFN-γ, IL-6, and TNF-α. (n=8; per group). B and C. Predicted relationships using IPA pathway connection tool. D. scRNA-seq t-SNEs expression of IFN- γ, IL-6, and TNF-α in cells derived from the lungs. In panel E: Studying the role of IL-6 in the activation of chemokines. MNCs were isolated from groups of 5 naïve mice and cultured in the presence of IL-6, LPS or IL-6+LPS. Eighteen hours later, the cells were harvested and examined for the expression of CCL3, CXCL2, and CXCL3 using RT-qPCR. Mouse 18S primers were used as an internal control. *p<0.05, **p<0.01, ***p<0.0002, ****p<0.0001.