FIG. 6.

Regulation of SeV-induced genes by IRF-3 and altered expression of SeV-regulated genes in U4C cells and U4C.2 cells, which express high levels of IRF-3. (A and B) Microarray data for the induction (n-fold) of select SeV-regulated genes in U4C and U4C.2 cells. Genes are grouped as follows: IRF-3 enhanced, genes with augmented expression in IRF-3-overexpressing cells (i.e., induced more strongly in U4C.2 cells); IRF-3 neutral, genes unaffected by cellular IRF-3 levels (induced at equivalent levels in both cell lines); IRF-3 repressed, SeV-induced genes negatively regulated by IRF-3 (induced in U4C but not U4C.2 cells). Also shown are quantitative RPA analyses of follistatin (C), NOXA (D), and A20 (E) mRNA induction in U4C and U4C.2 cells before or after SeV infection.