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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Pain. 2023 Jun 12;24(11):1980–1993. doi: 10.1016/j.jpain.2023.06.006

Figure 2: PirtCreF2rl1flox mice show attenuated mechanical hypersensitivity and facial grimacing in response to paclitaxel treatment.

Figure 2:

Baseline (BL) paw withdrawal thresholds and facial grimacing scores for male and female PirtCreF2rl1flox and control mice were obtained before paclitaxel treatment (4 mg/kg IP every other day for 4 days). (A) The control mice that received PTX treatment exhibited long-lasting mechanical allodynia that was significantly different from the PirtCreF2rl1+/+ Veh group on days 3, 7. 9, 13, 17, and 23 after the initial PTX injection. On the other hand, the PirtCreF2rl1flox mice treated with PTX show similar paw withdrawal thresholds to the PirtCreF2rl1+/+ Veh group. Pink asterisks denote significant differences between the PirtCreF2rl1+/+ PTX group and the PirtCreF2rl1+/+ Veh group. Gray plus signs denote significant differences between the PirtCreF2r1lflox Veh group and the PirtCreF2rl1+/+ Veh group. (B) Effect size was determined by calculating the cumulative differences between the baseline value and the experimental value at each time point. The PirtCreF2rl1+/+ PTX mice show a significantly higher effect size when compared to the PirtCreF2rl1+/+ Veh group. The effect sizes of the PirtCreF2rl1flox Veh and PirtCreF2rl1flox PTX groups were not significantly different from the PirtCreF2rl1+/+ Veh group. (C) The control mice that received PTX treatment also exhibited long-lasting facial grimacing that was significantly different from the PirtCreF2rl1+/+ Veh group on days 3, 7, 9, 13, and 17 after the initial PTX injection. However, both of the PirtCreF2rl1flox groups showed similar grimace scores to the PirtCreF2rl1+/+ Veh group. Pink asterisks denote significant differences between the PirtCreF2rl1+/+ PTX group and the PirtCreF2rl1+/+ Veh group. Black asterisks denote significant differences between the PirtCreF2rl1flox PTX group and the PirtCreF2rl1+/+ Veh group. (D) The PirtCreF2rl1+/+ PTX mice show a significantly higher effect size when compared to the PirtCreF2rl1+/+ Veh group. The effect sizes of the PirtCreF2rl1flox Veh and PirtCreF2rl1flox PTX groups were not significantly different from the PirtCreF2rl1+/+ Veh group. For the PirtCreF2rl1+/+ Veh group, n = 3 females and n = 3 males. For the PirtCreF2rl1+/+ PTX group, n = 4 females and n = 3 males. For the PirtCreF2rl1flox Veh group, n = 2 females and n = 4 males. For the PirtCreF2rl1flox PTX group, n = 3 females and n = 6 males. *p <0.05, **p<0.01, ***p<0.001, ****p<0.0001. For paw withdrawal threshold, repeated measures two-way ANOVA and Dunnett’s multiple comparisons test was used. For facial grimace scores, repeated measures two-way ANOVA with Dunnett’s multiple comparisons test was used. For effect size, one-way ANOVA with Dunnett’s multiple comparisons test was used. All group mean comparisons were made to the PirtCreF2rl1+/+ Veh group.