FIGURE 1.

Time-Interval PCA indicates time sensitive inflammatory markers of IC/BPS. Graphs of the sum of principal components indicating the extent to which each inflammatory mediator assayed perpetuated inflammation across the specified time interval (PC = principal component). (A–C) During the early time interval between 150 and 330 min following CYP injection, GRO-KC, MCP-1, IL-18, and IL-5 account for the most amount of variance in the acute inflammatory response characteristic of IC/BPS. (D–F) In the intermediate time interval between 330 and 510 min following CYP injection, GRO-KC and IL-5 continue to account for a high degree of variance in the inflammatory response. IL-6 and IFNγ also account emerge as inflammatory mediators with a high degree of variance. (G–I) The late phase of the inflammatory response between 510 and 690 min is characterized by inflammation mediated by IL-5, IL-6, and IFNγ. CYP, cyclophosphamide; IC/BPS, interstitial cystitis and bladder pain syndrome; PCA, principal component analysis.