TABLE 2.
Constructs and PCR primers used in construction of full-length clones with C-terminal deletions in the sequence of VP2 protein
| FL clones | Sequencea | Nucleotide and amino acid borders of deleted sequence (ORF3/VP2) |
|---|---|---|
| pF3C(259-288) | cctatagcttaagcgTTGATTTGTGTATGAGTAAGG | 259-288/87-96 |
| pF3C(229-288) | cctatagcttaagcgAAAACCAGCAGCACGTGC | 229-288/77-96 |
| pF3C(199-288) | cctatagcttaagcgCTGGAGGGGGCCTTGTACCCTTTG | 199-288/67-96 |
| pF3C(169-288) | cctatagcttaagcgAATTTTGTTAAACTGCTCTAC | 169-288/57-96 |
a
The sequences of the oligonucleotides complementary to nt 7605 to 7619 of the FCV genome sequence (in pRAfI) are shown in lowercase. The sequences of the oligonucleotides located immediately upstream of the engineered deletion are in uppercase.