TABLE 3.
Replication and reversion of the vF3Nterm mutant
| FCV FL cDNA clones and recovered viruses | ORF3 nucleotide sequence (codons 6-8)a | Encoded amino acid (codon 7) |
|---|---|---|
| pR6 | GGCTTGATT | Leu |
| pF3Nterm | GGCTAGATT | AMB |
| vF3Nterm (passage 4) | GGCCAGATT | Gln |
| vF3Nterm (passage 4, sample 1) | GGCCA/TGATT | Gln/Leu |
| vF3Nterm (passage 4, sample 2) | GGCCTGATT | Leu |
a
Comparison of the 5′-end sequences of ORF3 determined for the vF3Nterm mutant at passages 4 and 5. Passage 5 is represented by two virus samples collected independently from different wells of a multiwell plate at 24 h postinfection. The ORF3 codon corresponding to position 7 of the VP2 amino acid sequence is underlined.