FIG. 2.

MVECs transduced with HIV-1 Nef and SNV-Nef vector virions expressing Nef. Primary isolated human brain endothelial cells were transduced with either HIV-1 LacZ, HIV-1 Nef, or SNV-Nef vector virions and then cultured on two-well chamber slides for 48 h. The cells were fixed with 2% paraformaldehyde and incubated with primary antibodies against Nef, and later with Cy2-conjugated secondary antibodies, after permeabilization. These results are representative of those from experiments performed in triplicate and repeated at least twice.