Fig. 1. Ethanol fails to displace radioligand binding to DAT (A), NET (B) and SERT (C).

In contrast, known inhibitors of these transporters displaced binding with Ki values in the nM range, as expected (see Table 1). Data shown are mean and SEM, N = 3 mice per assay. Where the error bar is not visible, it is obscured by the symbol. Note: Radioligand binding to DAT was performed on striatal homogenates using 2.7 nM [³H]WIN 35,428, unlabeled GBR 12909 at 11 concentrations from 0.01 nM to 1 mM and ethanol from 3.4 nM to 1.0 M. Radioligand binding to NET was performed on hippocampal homogenates using 1.7 nM [³H]nisoxetine, unlabeled reboxetine at 11 concentrations from 100 pM to 250 µM, and ethanol from 3.4 nM to 1.0 M. Radioligand binding to SERT was performed on hippocampal homogenates using 1.5 nM [³H]citalopram, unlabeled citalopram at 7 concentrations ranging from 5 pM to 5 µM and ethanol from 3.4 nM to 1.0 M. Cartoon created with Biorender.com.