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. Author manuscript; available in PMC: 2024 Oct 26.
Published in final edited form as: Cell. 2023 Oct 6;186(22):4818–4833.e25. doi: 10.1016/j.cell.2023.09.007

Figure 3. Cryo-EM reconstruction of duck MXRA8 bound to WEEV.

Figure 3.

A. Cryo-EM density map of WEEV-VLP bound to duck MXRA8 at 4.74 Å resolution. WEEV-E1, WEEV-E2, and MXRA8 are colored as light-blue, dark-blue, and violet, respectively. Rotational symmetries along the 2-fold, 3-fold, and 5-fold axes are displayed with white numbers. B. Map of WEEV-VLP bound to duck MXRA8. Asymmetric unit contains the entire quasi 3-fold spike (q3), and a single icosahedral spike (i3) E1-E2 heterodimer. Shown are two views of the asymmetric unit, a side view (left, parallel with the viral membrane) and a top-down view (right, perpendicular with the viral membrane). Local resolution of map is colored from blue (4.25 Å) to white (5.25 Å) to red (6.25 Å). Density maps are viewed at contour level = 0.26 (0.96σ). C. Surface diagram of MXRA8 at site 3, detailing the three unique viral E1-E2 heterodimer contacts, wrapped (light gray), intraspike (gray), and interspike (dark gray). D. Surface diagram of MXRA8 at site 3 and interacting E1-E2 heterodimers, termed: E1-E2-wrapped, E1-E2-intraspike, or E1-E2-interspike. MXRA8 D1: light magenta; MXRA8 D2: dark magenta; E1 domain I: light gray; E1 domain II: medium-gray; E1 domain III: dark-gray; E1-TM (black); E2 A domain: light-cyan; E2 β-linker: medium-cyan; E2 B domain: dark-cyan; E2 C domain: medium-blue; E2-TM: dark-blue. See also Fig S5, S6, and Tables S2–S4.