FIG. 1.

HIV-specific responses against Gag, Env, and Nef as determined by the ELISPOT assays. Freshly isolated PBMC were tested against pooled Gag, Env, or Nef peptides corresponding to clade A and D consensus sequences. (A) Individual responses against Gag, Env, or Nef proteins alone and all three proteins combined for either clade A or D were calculated as the percentage of total volunteers tested (n = 177). (B) Frequencies of responses expressed as mean SFC per 10⁶ PBMC and SD (error bars) were reported for each protein tested.