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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Adv Biol (Weinh). 2023 May 4;7(10):e2200202. doi: 10.1002/adbi.202200202

Figure 1.

Figure 1.

The effect of different methods for fixing cultured cells on mitochondrial morphology determined by TEM. A–D) Live cells were scraped directly into McDowell Trump’s fixative. E,G) Cells were fixed for 5 min and then scraped in McDowell Trump’s fixative. F,H) Cells were fixed for 10 min and then scraped in McDowell Trump’s fixative. I,K) Cells were fixed for 5 min in McDowell Trump’s fixative without scraping. J,L) Cells were fixed for 10 min in McDowell Trump’s fixative without scraping. M,O) Cells were fixed for 30–60 min in McDowell Trump’s fixative without scraping. N,P) Cells were fixed for 24 h in McDowell Trump’s fixative without scraping. Cells were treated with insulin for 2 h before fixation.