Figure S3.
STIM-mediated Bcl2a1 expression in induced GC B cells decreases apoptosis. Related to Fig. 5. (A) Representative flow cytometry of iGB cells obtained from control B1-8high and Stim1/2 BKO B1-8high B cells. (B) Igκ-negative iGB cells obtained from control B1-8high and Stim1/2 BKO B1-8high B cells were stimulated with or without 4 µg/ml NP-Ficoll for 16 h. Percentages of Annexin V+ cells in iGB cells are shown as apoptotic cells. (C) iGB cells obtained from control and Stim1/2 BKO mice were stimulated with anti-IgG for 16 h. qRT-PCR of mRNA encoding Bcl2a1 or Mcl1 were assessed in iGB cells after stimulation with anti-IgG, normalized to the expression of β-actin. Data are shown as relative expression to unstimulated control. Data are presented as mean ± SEM of six measurements. Data are pooled from three independent experiments. NS, not significant. **, P < 0.01 (two-way ANOVA).