FIG. 1.

BAF57 is expressed in the luminal epithelia. Mouse prostates were resected and the individual lobes (ventral lobe [VL, lane 3], dorsolateral lobe [DLL, lane 4], and anterior lobe [AL, lane 5]) were isolated and prepared for either immunoblotting or immunohistochemistry as described in the text. (A) BAF57 and AR were detected by immunoblotting. LNCaP cell lysate (lane 1) and BT549 cell lysate (lane 2) were used as positive and negative controls, respectively. (B) Tissue from mouse prostate was sectioned and stained by standard immunohistochemistry techniques with no primary antibody control (left panels) or BAF57 (right panels). Antibody reactivity was visualized with diaminobenzidene chromogen, and nuclei were counterstained with hematoxylin. Magnifications, ×20 (top panels) and ×40 (bottom panels).