FIG.5.

BAF57 action is dependent upon SWI/SNF ATPase activity. (A) BT549 cells (2 × 10⁵) were transfected with 0.5 μg of pARR2-Luc reporter, 0.25 μg of pTK-Renilla luciferase, 0.5 μg of pSG5AR, and 2.25 μg of pBabe-WTBAF57, 1 μg of dominant-negative BRM (dnBRM), or parental vector (to 4.5 μg of total DNA) as indicated. Cells were treated posttransfection with 0.1% EtOH or 0.1 nM DHT for 24 h. Cells were harvested and lysed, and the dual luciferase activity was measured. Ligand-activated AR activity was set to 100, and relative luciferase activity and standard deviations are shown. ✽, P < 0.05. (B) BT549 cells were transfected with 0.5 μg of SG5AR, 0.5 μg of pGreenLantern GFP, and 1 μg of vector (lane 1) or dnBRM (lane 2) expression plasmid. Cell lysates were obtained, and immunoblot analysis was performed for AR and GFP. (C) SW13 cells (2 × 10⁵) were transfected with 0.5 μg of pARR2-Luc reporter, 0.25 μg of pTK-Renilla luciferase, 0.5 μg of pSG5AR, and 2.25 μg of pBabe-WTBAF57, 1 μg of BRM, and parental vector (to 4.5 μg of total DNA) as indicated. Posttransfection, cells were treated with 0.1% EtOH or 0.1 nM DHT for 24 h. Cells were harvested, lysed, and dual luciferase activity was measured. Ligand-activated AR activity was set to 100, and relative luciferase activity and standard deviations are shown.