FIG. 8.

BAF57 facilitates non-p160 AR coactivator function. (A) BT549 cells (2 × 10⁵) were transfected with 0.5 μg of pARR2-Luc reporter, 0.25 μg of pTK-Renilla luciferase, 0.5 μg of pSG5AR, and 0.01 to 2.25 μg of pBabe-WTBAF57 in the absence (▪) or presence of 1 μg of pSG5ARA70 (▨). As described in Materials and Methods, all transfections were brought to a total 4.5 μg by using empty vector. Posttransfection, cells were treated with 0.1% EtOH or 0.1 nM DHT for 24 h. Cells were harvested and lysed, and the luciferase activity was measured. The ligand-activated AR activity was set to 100 (data not shown), and the average relative luciferase activity and standard deviations are shown (P < 0.05). (B to D) BT549 cells (2 × 10⁵) were transfected with 0.5 μg of pARR2-Luc reporter, 0.25 μg of pTK-Renilla luciferase, 0.5 μg of pSG5AR, and 2.25 μg of pBabe-WTBAF57, vector, or the indicated coactivator (pSG5ARA55 and pFLAG-Ubc9) or mutant (pcDNA3-ARA70N2) expression plasmid (1 μg). Posttransfection, cells were treated with 0.1% EtOH or 0.1 nM DHT for 24 h. Cells were harvested and lysed, and luciferase activity was measured. The ligand-activated AR activity in the absence of BAF57 was set to 100, and the average relative luciferase activity and standard deviations are shown (P < 0.05).