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Affinity of the cHS4 insulator to LIS-extracted nuclear matrices. The in vitro S/MAR reassociation assay with a [35S]ATP-labeled 2.4-kb cHS4 sequence shows no affinity of the insulator to the nuclear matrix. As a positive control, the 95% binding S/MAR-E sequence was used, and the pTZ-vector backbone served as negative control. I, input; P, pellet fraction; S, supernatant.
