FIG. 6.

Ligand-specific displacement of PR in the presence of antagonists during chromatin remodeling in vitro. Pull-down assays were performed either with chromatin (lanes 1 to 6) or with DNA (lanes 7 to 12), in the presence of purified wild-type SWI/SNF (lanes 1 to 12), with (lanes 1, 3, 5, 7, 9, and 11) or without (lanes 2, 4, 6, 8, 10, and 12) ATP, and with PRB bound to agonist R5020 (lanes 1, 2, 7, and 8), antagonist RU486 (lanes 3, 4, 9, and 10), or antagonist ZK98299 (lanes 5, 6, 11, and 12). The bound proteins were probed by Western blotting to detect PR (upper panels, lanes 1 to 12) or BRG1 (lower panels, lanes 1 to 12). No displacement of PRB-ZK98299 in the presence of ATP was observed.