TABLE 1.
Yeast strains used in this study
| Strain backgrounda | Strain name | Genotype or relevant genotype | Source |
|---|---|---|---|
| a | KBY211 | MATα ste20::ADE2 cla4::LEU2 + YCpTRP1-cla4-75ts | 28 |
| a | PPY866 | MATα FUS1::FUS1-lacZ::LEU2 ste20-1::TRP1 ste5::ADE2 ste4::ura3FOA | 56 |
| a | PPY913 | MATaFUS1::FUS1-lacZ::LEU2 ste20-3Δ::TRP1 | 31 |
| a | PPY1249 | MATaADE2 ste20-1::TRP1 | This study |
| a | PPY1287 | MATassk1::HIS3 ste20-1::TRP1 | This study |
| a | PPY1456 | MATaFUS1::FUS1-lacZ::LEU2 ste20-3Δ::TRP1 nbp2Δ::kanR | This study |
| a | PPY1691 | MATaFUS1::FUS1-lacZ::LEU2 ste20-1::TRP1 ste5::ADE2 hog1::hisG | This study |
| b | PPY1209 | MATahis3::hisG leu2::hisG trp1::hisG ura3-52 ste20-1::TRP1 | 31 |
| c | PPY760 | MATaade2 his3 leu2 trp1 LYS2::lexAop-HIS3 URA3::lexAop-lacZ far1::ADE2 | 10 |
| d | DLY1 | MATabar1 ade1 his2 leu2 trp1 ura3 | 8 |
| d | DLY4000 | MATabar1 ade1 his3 leu2 trp1 ura3 BEM1-myc12::HIS3 | 8 |
| d | PPY1341 | MATabar1 ade1 his2 leu2 trp1 ura3 FUS1::FUS1-lacZ::HIS2 ste20::TRP1 bem1::ura3::LEU2 | This study |
| d | PPY1343 | MATabar1 ade1 his2 leu2 trp1 ura3 FUS1::FUS1-lacZ::HIS2 ste20::TRP1 | This study |
| d | PPY1356 | MATabar1 ade1 his2 leu2 trp1 ura3 NBP2-myc13::kanR | This study |
| d | PPY1415 | MATabar1 ade1 his2 leu2 trp1 ura3 FUS1::FUS1-lacZ::HIS2 ste20::TRP1 npb2Δ::kanR | This study |
| e | PPY1646 | MATaleu2 trp1 ura3 ssk2::LEU2 ssk22::LEU2 ste20Δ-3::TRP1 | This study |
| e | PT2α | MATα hom3 ilv1 can1 | 56 |
a
Strain backgrounds: a, W303 (ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1); b, Σ1278b; c, S288C; d, 15Dau; e, other.