TABLE 3.
Effects of Bem1 SH3 domain mutations on interaction with Ste20
| DBD fusiona | Interactionb with the following AD-BEM1 (1-551) fusionc:
|
|||
|---|---|---|---|---|
| Vector | Wild type | W108A (SH3-1) | W192A (SH3-2) | |
| Ste20 1-939 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| Ste20 1-499 | 0.1 ± 0.1 | 15.0 ± 6.6 | 21.3 ± 5.9 | 0.1 ± 0.1 |
| Ste20 434-499 | 0.1 ± 0.1 | 298.5 ± 69.2 | 430.1 ± 73.6 | 0.4 ± 0.6 |
| Cdc24 | 0.1 ± 0.1 | 143.6 ± 29.6 | 176.3 ± 31.4 | 47.2 ± 6.2 |
a
Plasmids pDH37, pB20N2, pB20BC, and pBTM-CDC24. DBD, DNA binding domain.
b
Expressed as mean β-galactosidase units ± standard deviation (n = 6) in the two-hybrid tester strain PPY760.
c
Plasmids pGADXP, pXP-BEM1, pXP-BEM1-W108A, and pXP-BEM1-W192A.