FIG. 2.

Adr1 and Cat8 cooccupy coregulated promoters. Sequential ChIP was performed on cells with myc-tagged Adr1 and hemagglutinin-tagged Cat8 and derepressed for 6 h. A/C, sample was precipitated for bound Adr1 and then bound Cat8; C/A, sample was precipitated for bound Cat8 and then bound Adr1. PCR using primers to the indicated promoters was performed on input (whole-cell extract) and ChIP samples. As positive controls, each factor was subjected to sequential precipitations with the same antibody (lanes C/C and A/A are Cat8 and Adr1 precipitations, respectively). As negative controls, each factor was subjected to a single precipitation followed by a mock sequential precipitation using protein A-Sepharose only (lanes C/- and A/-).