FIG. 4.

Adr1 binding alone assists Cat8 activation of ADH2. In-gel assays for ADH activity are shown for strains that are Δadr1CAT8 (TYY497) and Δadr1 Δcat8 (TYY495) with one of the following plasmids: empty vector pRS314 (-) (42); pJS21, which expresses the first 172 amino acids of Adr1, containing the entire DNA-binding domain but lacking the major activation domain (BD) (43); or pJS20, which expresses wild-type full-length Adr1 (ADR1) (43). Activity levels in Miller units from an integrated β-Gal reporter (16) in the same strains are shown below. In-gel activity assays were done with extracts prepared after 6 h of derepression in 0.05% glucose; reporter assays were done with cells derepressed for 24 h. The upper ADHI band from constitutively expressed ADH 1 served as a loading control for the in-gel activity assay.