Table 1.
Melting temperature of the parent enzyme (L3-35A) and its mutants as determined by thermal shift assay (a) and circular dichroism (b).
| Enzyme | Tm (°C)a | Tm (°C)b |
|---|---|---|
| PnFPOX (WT) | 53.2 ± 0.2 | 53.5 ± 0.1 |
| L3-35A | 52.3 ± 0.2 | 52.9 ± 0.1 |
| D02 | 53.1 ± 0.5 | 54.8 ± 0.1 |
| C16 | 55.2 ± 0.1 | 55.0 ± 0.1 |
| X01 | 54.1 ± 0.1 | 54.2 ± 0.1 |
| X02A | 60.0 ± 0.4 | 60.1 ± 0.1 |
| X04 | 55.2 ± 0.3 | 55.0 ± 0.1 |
| X07 | 55.3 ± 0.4 | 55.4 ± 0.1 |
| X02B | 60.2 ± 0.7 | 60.6 ± 0.1 |
| X02C | 64.0 ± 0.2 | 63.3 ± 0.1 |
The melting temperature of the wild type enzyme (PnFPOX) is also provided as reference.