FIG. 8.
Effect of siRNA-mediated knockdown of TRAF4 on acute TNFα-induced ERK1/2 activation and p47phox phosphorylation in HMEC-1. (A) Immunoblotting for TRAF4 expression and ERK1/2 activation after anti-TRAF4 siRNA treatment. HEK293 cells, which do not express TRAF4 endogenously (6a), were used as a negative control for TRAF4 expression. (B) Assessment of serine phosphorylation of p47phox after knockdown of TRAF4. The same blot was reprobed with an anti-p47phox antibody, while a parallel membrane was immunoblotted (IB) for p22phox. IP, immunoprecipitated.