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Quantitation of mean overhang sizes from the overhang protection assay. The gel (A) was first overlaid with a grid of boxes (B) and then quantitated (C). The values after subtracting the background (D) correspond to the term ODi, while the values after dividing the signal by the size in nucleotides (E) correspond to the signal ODi/Li in the formula Σ(ODi)/Σ(ODi/Li) used to calculate the final mean sizes. See text for details.
