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. 2005 Mar;25(6):2158–2168. doi: 10.1128/MCB.25.6.2158-2168.2005

FIG. 6.

FIG. 6.

Cells maintain their 3′ overhangs during their extended life span. (A) Growth curve of BJ fibroblasts ectopically expressing HPV E6 only, E7 only, or pLXSN (vector-only control). BJs were infected at PD 78 with retrovirus containing E6 (filled squares), E7 (filled triangles), or vector only (diamonds). After selection, cells were cultured until no cell number increase in 4 weeks was observed. (B) TRAP analysis of BJ cells ectopically expressing E6, E7, E6 and E7, L-Tg, or hTERT. Nonradioactive TRAP assays were performed by using whole-cell lysates from 2,500 cells. ITAS represents the 36-bp internal TRAP assay standards. (C) Weighted mean sizes of overhangs from the overhang protection assay from BJ fibroblasts ectopically expressing E6 or E6 and E7 (E6/E7) at different PD. Results are representative of at least three independent experiments. Error bars represent one standard deviation.