TABLE 3.
Frequencies of SB integration events within intragenic regions of mouse cellsa
| Genomic location | % of integrationsb
|
||||
|---|---|---|---|---|---|
| Random |
SB
|
AAVf | |||
| In mouse liverc | In NIH 3T3 cellsd | Alle | |||
| In RefSeq genes | 26.0 | 31.2 | 32.9 | 31.9 | 53.1 |
| 5 kb upstream of genes | 3.2 | 5.1 | 6.1 | 5.5 | 25.8 |
| ±5 kb from transcription start site | 6.4 | 10.5 | 9.2 | 10.0 | 43.9 |
| ±5 kb from CpG islands | 6.4 | 9.5 | 8.7 | 9.2 | 49.3 |
a
Compared with frequencies of AAV integrations in a recent study.
b
Integrations were compared to 10,000 computer-simulated random integrations and were analyzed by using a χ2 test for statistical significance.
c
All values in this group are distinguishable from random integration (P ≤ 0.01).
d
All values in this group (except for ±5 kb from CpG islands [P = 0.07]) are distinguishable from random integration (P ≤ 0.03).
e
The mouse liver and NIH 3T3 data sets were combined. All values in this group are distinguishable from random integration (P < 0.001).
f
From the work of Nakai et al. (39). All values in this group are distinguishable from random integration (P < 0.001).