TABLE 5.

Target site analysis for 1,309 unique genomic SB insertions^a

Nucleotide	No. of insertion sites with the indicated nucleotide at position:
	−5	−4	−3	−2	−1	T	A	+1	+2	+3	+4	+5	+6	+15
A	461	284	904	214	615	0	1,309	194	553	228	426	288	463	379
C	230	350	68	327	226	0	0	257	230	111	260	344	223	240
G	337	233	120	227	247	0	0	230	325	51	341	214	262	225
T	282	442	217	541	221	1,309	0	628	201	919	282	463	361	465
Consensusb	R	C	A	Y	A	T	A	T	R	T	G	Y	A	T

^aThe base composition of the 60-bp region encompassing the TA target dinucleotide (underlined) was determined and compared to that of 10,000 randomly selected genomic coordinates. The random nucleotide frequencies at each position were identical to the genome average: 30% for A, 20% for C, 20% for G, and 30% for T. Only positions at which significant deviation between the two groups was observed (P < 0.01 by the χ² test) are shown.

^bR stands for A or G; Y stands for C or T.