Fig. 4. Novel isoform diversity and classifications are associated with known developmental genes.

A PCA of gene expression across short-read RNA-Seq samples, colored by developmental stage. B Percentage of short RNA-Seq reads mapping to novel isoforms for each gene expressed in each developmental stage. C Number of significantly differentially expressed isoforms at each developmental stage transition. D Heatmap of expression levels for selected developmental genes, including the classes of isoforms found for each gene (known, NIC, NNC). The statistical significance and direction of differential gene expression across developmental stage transitions are presented with arrows. E Detailed dynamic expression of selected known and novel isoforms of three representative developmental genes: DNMT1, PADI6 and FOXO3. Source data are provided as a Source Data file.