Figure 2.

Classification of lung tissue cores according to emphysema severity. (A) Cores tissue percentage and surface density were determined based on microCT and used to define emphysema severity groups among COPD cores, separated by a dashed line (lower panel): mild COPD cores, moderate COPD cores and severe COPD cores, in addition to control cores (CT). A transversal section microCT of one representative core per group is presented (upper panel). Each point corresponds to individual core data. (B) Terminal bronchioles (TB) count in controls and COPD cores. Each point corresponds to individual core data, ***p<0.0005 vs controls (CT) by a linear mixed effects regression models including cores disease subgroups, age, and gender as fixed effect and patient as random effect (C) Volcano plots of differential gene expression data in mild COPD cores, moderate COPD cores and severe COPD cores as compared with controls. Top significant genes are labeled. Each point corresponds to individual core data. (D) PCA analysis with component 1 and 2 plotted. Each point corresponds to individual core data. (E) Venn diagram showing numbers of differentially expressed genes in each cores group comparison with controls.