Table 2.
Comparison of genes expression and pathways enrichment scores involved in innate mucosal defenses and (IgA-mediated) adaptive immunity.
| COPD, mild cores vs CT | COPD, moderate cores vs CT | COPD, severe cores vs CT | ||||
|---|---|---|---|---|---|---|
| Genes | LogFC | adj p-value | LogFC | adj p-value | LogFC | adj p-value |
| Defensins | ||||||
| DEFA4 | -2.66 | 0.06 | -3.34 | <0.001 | -3.02 | 0.002 |
| DEFA3 | -3.34 | 0.03 | -4.18 | <0.001 | -3.77 | 0.001 |
| DEFA1 | -2.28 | 0.16 | -2.53 | 0.003 | -2.3 | 0.02 |
| DEFB4A | -2.97 | 0.11 | -1.09 | 0.315 | -3.04 | 0.01 |
| Mucins | ||||||
| MUC5AC | 2.9 | 0.12 | 4.72 | <0.001 | 1.71 | 0.29 |
| MUC5B | 1.14 | 0.49 | 2.02 | 0.018 | -0.52 | 0.72 |
| SPDEF | 1.45 | 0.09 | 1.95 | 0.001 | 0.34 | 0.67 |
| MUC13 | 3.82 | 0.008 | 4.44 | <0.001 | 1.43 | 0.27 |
| MUC15 | 1.4 | 0.01 | 1.02 | 0.003 | 0.525 | 0.25 |
| MUC20 | 1.64 | 0.06 | 1.81 | 0.001 | 0.157 | 0.87 |
| Antimicrobial peptides | ||||||
| SLPI | 0.91 | 0.06 | 0.58 | 0.06 | 0.27 | 0.52 |
| TLR5 | 0.54 | 0.17 | 0.4 | 0.12 | 0.51 | 0.07 |
| Genes involved in IgA-mediated adaptive immune response | ||||||
| PIGR | 0.64 | 0.16 | 0.68 | 0.008 | 0.13 | 0.76 |
| MHC Class I genes | ||||||
| HLA-G | 3.33 | 0.002 | 0.756 | 0.345 | 2.76 | <0.001 |
| MHC Class II genes | ||||||
| HLA-DQA1 | 0.69 | 0.26 | 0.86 | 0.02 | 0.84 | 0.04 |
| HLA-DRA | 0.39 | 0.30 | 0.47 | 0.03 | 0.60 | 0.01 |
| HLA-DOB | 0.15 | 0.82 | 0.70 | 0.02 | 0.65 | 0.06 |
| HLA-DOA | 0.39 | 0.38 | 0.56 | 0.03 | 0.50 | 0.09 |
| Pathways | Enrichment score | adj p-value | Enrichment score | adj p-value | Enrichment score | adj p-value |
| Immune network for IgA production | 0.63 | 0.02 | 0.70 | 0.001 | 0.73 | <0.001 |
| IL-17 signaling pathway | 0.52 | 0.08 | 0.59 | 0.02 | 0.53 | 0.02 |
| Cytokine-Cytokine receptor interaction | 0.49 | 0.06 | 0.52 | 0.09 | 0.55 | <0.001 |
| TGF-beta signaling pathway | 0.51 | 0.31 | 0.50 | 0.31 | 0.50 | 0.04 |
| Toll-like receptor signaling pathway | 0.44 | 0.39 | 0.20 | 0.99 | 0.50 | 0.04 |
| JAK-STAT signaling pathway | 0.48 | 0.13 | 0.41 | 0.87 | 0.55 | 0.002 |
| Th17 cell differentiation | 0.3 | 0.7 | 0.44 | 0.69 | 0.49 | 0.04 |
Results are presented as log2-transformed fold change (log2FC) of gene expression data and pathway enrichment scores between each COPD cores groups as compared with controls cores group and FDR adjusted p-value, calculated using a linear multiple regression analysis including gender and age as covariates. FDR<0.1 was considered significant. Statistically significant results are in bold.