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Absence of normal Rhpn2 mRNA in Rhpn2−/− thyroid. (A) Exons 5 to 8 (filled boxes) of the mouse Rhpn2 gene in wild-type and mutant alleles and localization of primers P5, P7a, P7b, and P8 (arrowheads). (B) Electrophoresis of PCR products generated with the indicated primers on cDNA isolated from Rhpn2+/+ and Rhpn2−/− thyroids. H, hypoxanthine phosphoribosyltransferase primers. Right margin, lengths of DNA fragments generated by PCR.
