Skip to main content
. 2023 Oct 14;26(11):108170. doi: 10.1016/j.isci.2023.108170

Figure 2.

Figure 2

Silencing MAN1A1 increases high-mannose synthesis and inhibits decidualization of endometrial stromal cells

(A–D) HESCs were transfected with MAN1A1-siRNA and MAN1A1-cDNA, and mRNA and protein levels of MAN1A1 were detected by qPCR and western blot.

(E–G) Western blot analysis of MAN1A1, PRL (prolactin), and insulin-like growth factor-binding protein 1 (IGFBP-1) expression and Lectin blot analysis of high-mannose expression in HESCs after artificial decidua induction (ID) with MPA and dbcAMP, followed by transfection with MAN1A1-siRNA and MAN1A1-cDNA.

(H–J) Immunofluorescent staining of MAN1A1 (green), high-mannose (red), decidualization markers PRL (green), and IGFBP-1 (green) in HESCs after ID or uninduced control, followed by transfection with MAN1A1-siRNA or cotransfection with MAN1A1-cDNA. Golgi apparatus was labeled by Golgi-Tracker Red. Nuclei were stained with DAPI (blue). Scale bars, 20 μm ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.