Figure 3.
Increased IncNEAT1 level in the decidual tissues of miscarriage patients
(A) Integrative analysis of upregulated and downregulated lncRNAs in the serum of infertile patients compared with healthy women, and MAN1A1-correlated lncRNAs by Venn diagram.
(B) Intersection of lncRNAs (fold change >2) in correlation with MAN1A1 by volcano plot. lncNEAT1: para-nuclear assembled transcript 1.
(C) GO enrichment graph for upregulated lncRNAs by LncSEA database.
(D) Expression and localization of lncNEAT1 in human tissues by LncSEA database.
(E) qPCR analysis of lncNEAT1 level in the decidual tissues of early pregnancy and miscarriage.
(F) Pearson correlation analysis of lncNEAT1 and MAN1A1. Pearson R = 0.52; p = 0.04; n = 16. Linear regression: y = −0.2274x + 1.633; r2 = 0.2604.
(G) lncNEAT1 level in the nucleus and cytoplasm of HESCs by PCR.
(H and I) Fluorescence in situ hybridization (FISH) analysis of lncNEAT1 (red) in HESCs (H) and endometrial tissues (I). Nuclei are stained with DAPI (blue). Scale bars, 20 μm ∗p < 0.05.