REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-MUC1-C	Thermo Fisher Scientific	Cat # MA5-11202; RRID:AB_11000874
Anti-ZEB1	Cell Signaling Technology	Cat #3396; RRID:AB_1904164
Anti-TWIST1	Abcam	Cat #ab50887; RRID:AB_883294
Anti-BMI1	Cell Signaling Technology	Cat #6964; RRID:AB_10828713
Anti-MYC	Abcam	Cat #ab32072; RRID:AB_731658
Anti-SOX2	Cell Signaling Technology	Cat #3579; RRID:AB_2195767
Anti-β-actin	Sigma-Aldrich	Cat #A5441; RRID:AB_476744
Anti-GLUT1	Abcam	Cat #115730; RRID:AB_10903230
anti-HK2	Cell Signaling Technology	Cat #2867; RRID:AB_2232946
anti-SDHD	NOVUS Biologicals	Cat #NBP2-83506
anti-cytochrome c	Proteintech	Cat #10993-1-AP; RRID:AB_2090467
anti-ND1	Proteintech	Cat #19703-1-AP; RRID:AB_10637853
anti-COX2	Proteintech	Cat #55070-1-AP; RRID: AB_10859832
anti-TFAM	Cell Signaling Technology	Cat #8076S; RRID:AB_1094911
anti-TFB2M	Proteintech	Cat #24411-1-AP; RRID:AB_2879530
anti-mTERF3	Abcam	Cat #230232
anti-SOD2	Cell Signaling Technology	Cat #13141; RRID: AB_2636921
anti-PRDX3	Proteintech	Cat #10664-1-AP; RRID: AB_2284207
Bacterial and virus strains
Lentiviral particles for MUC1 shRNA	This paper	N/A
Lentiviral particles for MYC shRNA	This paper	N/A
Lentiviral particles for MUC1 shRNA#2	This paper	N/A
Lentiviral particles for control shRNA	This paper	N/A
Chemicals, peptides, and recombinant proteins
Lipofectamine 3000 Reagent	Invitrogen	Cat #L3000008
Trizol Reagent	Invitrogen	Cat #15596018
Puromycin	InvivoGen	Cat #ant-pr-1
Geneticin	Invitrogen	Cat #10131035
GO-203	This paper	N/A
2-DG	Selleck	Cat #S4701
Doxycycline	Millipore Sigma	Cat #24390-14-5
TrypLE Express	Thermo Scientific	Cat #12604013
TRIzol	Invitrogen	Cat #15596-018
Halt™ Protease Inhibitor Cocktail (100X)	Thermo Fisher Scientific	Cat #78430
Dynabeads Protein G	Thermo Fisher Scientific	Cat #10003D
Heparin Solution	Stemcell Technologies	Cat #ST-07980
Critical commercial assays
Power SYBR Green PCR Master Mix	Applied Biosystems	Cat #4367659
High Capacity cDNA Reverse Transcription Kit	Applied Biosystems	Cat #4368814
DNeasy Blood & Tissue Kit	QIAGEN	Cat #69504
MammoCult Human Medium Kit	Stemcell Technologies	Cat #ST-05620
AlamarBlue Cell Viability Reagent	Thermo Scientific	Cat #DAL1100
PureLink HiPure Plasmid DNA Purification Kit	Invitrogen	Cat #K210007
TruSeq Stranded mRNA Kit	Illumina	Cat #20020594
Glucose Uptake-Glo Assay	Promega	Cat #J1341
Luminescent ATP Detection Assay Kit	Abcam	Cat #ab113849
MitoTracker Green	Cell Signaling Technology	Cat #9074
MitoSOX Red	Thermo Fisher Scientific	Cat #M36008
MitoProbe JC-1 Assay Kit	Thermo Fisher Scientific	Cat #M34152
Zombie Aqua™	BioLegend	Cat #423101
MycoAlert Mycoplasma Detection Kit	Lonza	Cat #LT07-118
Deposited data
RNA-seq	NCBI GEO	GSE222377
scRNA-seq	NCBI GEO	GSE230308
Experimental models: Cell lines
BT-549	ATCC	CVCL_1092
MDA-MB-436	ATCC	CVCL_0623
HEK293T	ATCC	CVCL_0063
BT-549/tet-CshRNA	This paper	N/A
BT-549/tet-MUC1shRNA	This paper	N/A
BT-549/CshRNA	This paper	N/A
BT-549/MUC1shRNA	This paper	N/A
MDA-MB-436/tet-CshRNA	This paper	N/A
MDA-MB-436/tet-MUC1shRNA	This paper	N/A
BT-549/tet-MYCshRNA	This paper	N/A
Experimental models: Organisms/strains
NSG mice	Jackson Laboratory	N/A
Nude mice	Jackson Laboratory	N/A
Oligonucleotides
MUC1shRNA	SIGMA	TRCN0000122938
MYCshRNA	SIGMA	TRCN0000039642
MUC1shRNA#2	SIGMA	TRCN0000430218
CshRNA	Millipore Sigma	N/A
Primers for real-time PCR, see Table S1	This paper	N/A
Primers for ChIP-PCR, see Table S2	This paper	N/A
Software and algorithms
FlowJo v10.6.2	BD Biosciences	N/A
GraphPad Prism9	Dotmatics	N/A
R package	CRAN	N/A