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Mos1 Tnp forms four complexes with the Mos1 ITR. EMSA were performed using Tnp and ITR30 (lanes 1 to 4) or ITR70 (lanes 5 to 8) as probes. U, unbound DNA; C1, C2, C3, and C4, Tnp/ITR complexes; Mg, incubations in 5 mM MgCl2; E, incubations in 5 mM EDTA. Temperature conditions are indicated above the gels. Putative cleavage products in lane 7 are starred.
