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. 2005 Apr;25(7):2861–2870. doi: 10.1128/MCB.25.7.2861-2870.2005

FIG. 2.

FIG. 2.

Determination of the apparent molecular masses of Tnp/ITR complexes. (A) Native PAGE was performed using ITR30 as a probe and was run with prestained molecular standards. U, unbound DNA; C1, C2, C3, and C4, Tnp/ITR complexes. (B) UV cross-linking assays. PEC2 was assembled using either ITR30 (lanes 1 and 2) or BrdU ITR30 (lane 3) as a probe. Complexes were exposed to UV (lanes 2 and 3) or not (lane 1) and loaded onto SDS-PAGE gels with prestained molecular standards. (C) Glutaraldehyde fixation assays. PEC2s were assembled by using ITR30 as a probe. Complexes were subjected to glutaraldehyde treatment (lane 2) or not (lane 1) and then loaded onto SDS-PAGE gels with prestained molecular standards. (D) The apparent molecular mass of each complex as determined in panels A, B, and C. Values are means of at least three independent assays. The standard error is always less than 15% of the reported mean. The expected molecular mass of each complex is indicated on the right.