REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
OGT Rabbit Polyclonal antibody | Cell Signaling Technology | Cat#24083 |
OGA Rabbit Polyclonal antibody | Cell Signaling Technology | Cat#60406 |
O-GlcNac Mouse Monoclonal antibody | Cell Signaling Technology | Cat#12938 |
HIF-1α Rabbit Polyclonal antibody | Cell Signal Technology | Cat#36169 |
HIF-2α Rabbit Polyclonal antibody | Cell Signal Technology | Cat#71565 |
Actin Rabbit Polyclonal antibody | Cell Signal Technology | Cat#4970 |
IgG Rabbit Polyclonal antibody | Proteintech | Cat#30000-0-AP |
Ubiquitin mouse monoclonal antibody | Santa Cruz Biotechnology | Cat#sc-8017 |
Chemicals, peptides, and recombinant proteins | ||
DMSO | MedChemExpress | Cat#HY-100218A |
Erastin | MedChemExpress | Cat#HY-15763 |
sulfasalazine | MedChemExpress | Cat#HY-14655 |
propidium iodide | Invitrogen | Cat#P3566 |
C11 BODIPY 581/591 | Invitrogen | Cat#D3861 |
TRIzol Reagent | Invitrogen | Cat#15596026 |
Puromycin | Beyotime Biotechnology | Cat#ST551 |
Protein A/G sepharose beads | Santa Cruz Biotechnology | Cat#sc-2003 |
Critical commercial assays | ||
CCK8 reagent | Dojindo Molecular Technologies | Cat#CK04 |
PrimeScript™ RT Reagent Kit | Takara Bio | Cat#RR037A |
SYBR Premix Ex Taq™ kit | Takara Bio | Cat#DRR081A |
Dual-Glo Luciferase Assay system | Promega | Cat#E2920 |
Experimental models: Cell lines | ||
786-O | Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus | RRID: SCR_021982 |
769-P | Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus | RRID: SCR_021982 |
Caki-1 | Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus | RRID: SCR_021982 |
Experimental models: Organisms/strains | ||
BALB/c-nu mice | Beijing Vital River Laboratory Animal Technology | N/A |
Oligonucleotides | ||
sgNC: GCACTACCAGAGCTAACTCA | This paper | N/A |
sgOGT#1: GCTTAAGTTTCAGAGCCGTG | This paper | N/A |
sgOGT#2: TAGTTCGATAGCCCGCCTGT | This paper | N/A |
sgHIF2a: AGATGGGAGCTCACACTGTG | This paper | N/A |
Primer: HIF2a-F GAACGTCGAAAAGAAAAGTCTCG | This paper | N/A |
Primer: HIF2a-R CCTTATCAAGATGCGAACTCACA | This paper | N/A |
Primer: Actin-F GGGACCTGACTGACTACCTC | This paper | N/A |
Primer: Actin-R TCATACTCCTGCTTGCTGAT | This paper | N/A |
Recombinant DNA | ||
CRISPR-V2 vector | Addgene Sanjana et al.27 |
Cat#52961 |
pGL3-Basic vector | Addgene Chang et al.28 |
Cat#64784 |
Software and algorithms | ||
SPSS software v22.0 | IBM Corp | N/A |
GraphPad Prism v8.0 | GraphPad Software | N/A |