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. 2023 Oct 5;26(11):108148. doi: 10.1016/j.isci.2023.108148
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

OGT Rabbit Polyclonal antibody Cell Signaling Technology Cat#24083
OGA Rabbit Polyclonal antibody Cell Signaling Technology Cat#60406
O-GlcNac Mouse Monoclonal antibody Cell Signaling Technology Cat#12938
HIF-1α Rabbit Polyclonal antibody Cell Signal Technology Cat#36169
HIF-2α Rabbit Polyclonal antibody Cell Signal Technology Cat#71565
Actin Rabbit Polyclonal antibody Cell Signal Technology Cat#4970
IgG Rabbit Polyclonal antibody Proteintech Cat#30000-0-AP
Ubiquitin mouse monoclonal antibody Santa Cruz Biotechnology Cat#sc-8017

Chemicals, peptides, and recombinant proteins

DMSO MedChemExpress Cat#HY-100218A
Erastin MedChemExpress Cat#HY-15763
sulfasalazine MedChemExpress Cat#HY-14655
propidium iodide Invitrogen Cat#P3566
C11 BODIPY 581/591 Invitrogen Cat#D3861
TRIzol Reagent Invitrogen Cat#15596026
Puromycin Beyotime Biotechnology Cat#ST551
Protein A/G sepharose beads Santa Cruz Biotechnology Cat#sc-2003

Critical commercial assays

CCK8 reagent Dojindo Molecular Technologies Cat#CK04
PrimeScript™ RT Reagent Kit Takara Bio Cat#RR037A
SYBR Premix Ex Taq™ kit Takara Bio Cat#DRR081A
Dual-Glo Luciferase Assay system Promega Cat#E2920

Experimental models: Cell lines

786-O Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus RRID: SCR_021982
769-P Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus RRID: SCR_021982
Caki-1 Cell Technologies Resource Center in the University of Colorado Anschutz Medical Campus RRID: SCR_021982

Experimental models: Organisms/strains

BALB/c-nu mice Beijing Vital River Laboratory Animal Technology N/A

Oligonucleotides

sgNC: GCACTACCAGAGCTAACTCA This paper N/A
sgOGT#1: GCTTAAGTTTCAGAGCCGTG This paper N/A
sgOGT#2: TAGTTCGATAGCCCGCCTGT This paper N/A
sgHIF2a: AGATGGGAGCTCACACTGTG This paper N/A
Primer: HIF2a-F GAACGTCGAAAAGAAAAGTCTCG This paper N/A
Primer: HIF2a-R CCTTATCAAGATGCGAACTCACA This paper N/A
Primer: Actin-F GGGACCTGACTGACTACCTC This paper N/A
Primer: Actin-R TCATACTCCTGCTTGCTGAT This paper N/A

Recombinant DNA

CRISPR-V2 vector Addgene
Sanjana et al.27
Cat#52961
pGL3-Basic vector Addgene
Chang et al.28
Cat#64784
Software and algorithms
SPSS software v22.0 IBM Corp N/A
GraphPad Prism v8.0 GraphPad Software N/A