FIG. 4.

TLF but not TBP increased transcription from the NF1 promoter. (A) CAT assays showing that TLF stimulates transcription from an NF1 promoter fragment in a dose-dependent manner. (Top) Thin-layer chromatography (TLC) plate showing an increase in pNF1-CAT activity, measured by the acetylation of the FAST-CAT deoxy-green substrate, in response to TLF overexpression. (Bottom) Quantification of the effects of TLF on pNF1-CAT activity. The effects of TLF on pNF1-CAT were quantified by fluorometry and were compared to those for cells cotransfected with pNF1-CAT and eGFP. Error bars indicate the standard error of the mean (n = 6). Total DNA transfected in each condition was equalized to 6 μg by the addition of eGFP-C3. (B) TBP decreases pNF1-CAT activity in a dose-dependent manner. (Top) TLC plate showing a decrease in pNF1-CAT activity, measured by the acetylation of the FAST-CAT deoxy-green substrate, in response to TBP overexpression. (Bottom) Quantification of the effects of TBP on pNF1-CAT activity. The effects of TBP on pNF1-CAT were quantified by fluorometry and were compared to those for cells cotransfected with pNF1-CAT and eGFP. Error bars indicate the standard error of the mean (n = 6). Total DNA transfected in each condition was equalized to 6 μg by the addition of eGFP-C3.