FIG. 5.
Endogenous NF1 transcripts were downregulated in the absence of TLF. (A) RNase protection assay. Lane 1 contains RNA Century markers (Ambion) labeled with [33P]UTP. Lanes 2 and 3 contain undigested probes for β-actin and NF1, respectively. β-Actin was chosen as an internal control for the amount of RNA loaded in each lane. Lanes 4 and 5 contain RNA from either a TLF+/+ mouse brain or a TLF−/− mouse brain hybridized with probes for both β-actin and NF1. (B) The amount of NF1 RNA in each lane was quantified by phosphorimaging and normalized to the amount of β-actin RNA in each lane. The amount of RNA in TLF+/+ mice was normalized to 1; error bars indicate the standard error of the mean (n = 3) (P < 0.05).