FIG. 7.
TLF and TBP have reciprocal effects on a fragment of the c-fos promoter. (Upper panel) Quantification of the effects of TBP on c-fos CAT activity. The effects of TBP on c-fos CAT activity were quantified by fluorometry and compared to those for cells cotransfected with the c-fos CAT construct and eGFP. Error bars indicate the standard error of the mean (n = 6). Total DNA transfected in each condition was equalized to 6 μg by the addition of eGFP-C3. (Lower panel) Quantification of the effects of TLF on c-fos CAT activity. The effects of TLF on c-fos CAT activity were quantified by fluorometry and compared to those for cells cotransfected with the c-fos CAT construct and eGFP. Error bars indicate the standard error of the mean (n = 6).