Fig. 3.

Impact of CBS knocking down on CSE in MDA-MB-231 cells. Expression of CBS and CSE mRNA and protein levels were determined using qRT-PCR and Western blot 48 and 72 h post-transfection, respectively. CBS was efficiently knocked down on the (a) mRNA and (c) protein levels. KD of CBS increased (b) CSE mRNA and (c) protein levels when compared to mock cells. (d) H₂S levels appeared significantly lower in cells with both CBS and CSE KD than in cells with CBS KD alone or compared to mock. One-way ANOVA and unpaired Student's T test were performed. ** Significantly different from mock cells P < 0.01 ****significantly different from mock cells at P < 0.001.