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. 2005 Apr;25(7):2660–2672. doi: 10.1128/MCB.25.7.2660-2672.2005

FIG. 5.

FIG. 5.

pRB is recruited to E2F dependent promoters in response to E2F3 activation. (A) Transient induction of E2F targets upon E2F3 activation. Expression levels of the E2F targets EZH2, CDC2, and cyclin E1 (CCNE1) after E2F3 activation was assessed by quantitative PCR, normalized against GAPDH, and not induced (t = 0) was set as 1.0. (B) ER-E2F3 binds to E2F-responsive promoters and recruits pRB upon activation. Enrichment, relative to the input, of the EZH2, CDC2, and GAPDH promoters following E2F3, pRB, or Flag chromatin immunoprecipitation (ChIP) is shown.