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Effects of N-terminal truncation and mutation on MT localization. HeLa cells were examined 36 h after transfection with 96-476MT-GFP (A), 120-476MT-GFP (B), 130-476MT-GFP (C), 120-476(R127A)MT-GFP (D), 96-476(R127I)MT-GFP (E), 96-476(103-107A)MT-GFP (F), or 96-476(103-107A/R127I)MT-GFP (G). Fl, fluorescence microscopy; Nu, Hoechst nuclear staining.
