TABLE 1.
Actual and expected genotypes of double-heterozygous mating
| Parameter (n) | Result (%) for indicated genotypea
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| W/W | W/H | W/K | H/W | H/H | H/K | K/W | K/H | K/K | |
| Expected ratio | 1 (6.25) | 2 (13) | 1 (6.25) | 2 (13) | 4 (26) | 2 (13) | 1 (6.25) | 2 (13) | 1 (6.25) |
| No. of mice | |||||||||
| Males (398) | 32 (8.04) | 47 (11.81) | 29 (7.29) | 46 (11.56) | 93 (23.37) | 48 (12.06) | 25 (6.28) | 53 (13.32) | 25 (6.28) |
| Females (412) | 25 (6.07) | 68 (16.50) | 33 (8.01) | 51 (12.38) | 95 (23.06) | 44 (10.68) | 25 (6.07) | 40 (9.71) | 31 (7.52) |
| No. of MEFs (236) | 19 (8.05) | 31 (13.14) | 14 (5.93) | 24 (10.17) | 80 (33.89) | 22 (9.32) | 7 (2.97) | 24 (10.17) | 15 (6.36) |
| Total no. of mice and MEFs (1,046) | 76 (7.26) | 146 (13.96) | 76 (7.27) | 121 (11.57) | 268 (25.62) | 114 (10.89) | 57 (5.45) | 117 (11.19) | 71 (6.79) |
a
The first genotype represents Cdc25B, and the second genotype represents CDc25C. W, wild type; H, heterozygous; K, knockout.