TABLE 2.
Amounts and percentages of ToMV recovered from unconcentrated eluates of virus-amended mineral and organic WF soils
| Amt of ToMV added (ng)a | WF organic soil
|
WF mineral soil
|
Purified ToMV A405b | ||||
|---|---|---|---|---|---|---|---|
| A405b | ToMV recoveredc
|
A405b | ToMV recoveredc
|
||||
| Amt (ng) | % | Amt (ng) | % | ||||
| 1,500 | 1.88 | >100 | >2.00 | >100 | |||
| 1,000 | 1.28 | 80 | 8 | 1.43 | 83 | 8 | |
| 500 | 1.01 | 50 | 10 | 1.17 | 60 | 12 | |
| 250 | 0.68 | 40 | 16 | 0.85 | 5 | 18 | |
| 100 | 0.44 | 22 | 22 | 0.54 | 30 | 30 | 1.54 |
| 50 | 0.28 | 8 | 16 | 0.36 | 15 | 30 | 0.99 |
| 25 | 0.25 | 7 | 28 | 0.25 | 7 | 28 | 0.49 |
| 10 | 0.16 | 2 | 20 | 0.17 | 3 | 30 | 0.33 |
| 5 | 0.13 | 0.15 | 0.21 | ||||
| 1 | 0.12 | 0.13 | 0.14 | ||||
| 0 | 0.12 | 0.12 | 0.12 | ||||
| Mean | 17 | 22 | |||||
a
Purified ToMV was added at the concentrations indicated to 1 g of air-dried mineral soil or 100 mg of organic soil in a volume of 1 ml of 100 mM phosphate buffer (pH 7.0).
b
A405 values obtained by DAS-ELISA represent the mean of two wells.
c
The amount of virus recovered was determined by comparing DAS-ELISA A405 values of eluates to the absorbance of dilutions of purified ToMV (last column). The percentage of virus recovered was determined as follows: (amount [ng] of ToMV recovered/amount [ng] of ToMV added) × 100.